虹鳟(♀)×山女鳟(♂)杂交种胚胎及仔鱼发育的研究
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S965.2;S917

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中央级公益性科研院所基本科研业务费专项资金(2007HSYZX-YY-19;2009HSYZX-YY-10); 农业部淡水鱼类遗传育种和养殖生物学重点开放实验室课题(BZ2009-18)


A study on embryonic and larval development of hybrid F1 by Oncorhynchus mykiss (♀)× Oncorhynchus masou masou (♂)
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    摘要:

    利用人工繁育技术对虹鳟(♀)(Oncorhynchus mykiss)和山女鳟(♂)(Oncorhynchus masou masou)进行了种间杂交实验,并对杂交胚胎和仔鱼的发育进行了观察,通过观察,详细描述了其胚胎发育和仔鱼发育各个阶段的发育时序和形态特征。结果表明:在水温为4.3-9.2℃的条件下,杂交受精卵的有效发育积温达到206.01℃·d发眼,完成胚胎发育所需的有效积温为387.44℃·d,杂交鳟的胚胎发育速度介于双亲之间;初孵仔鱼,全身透明,血液循环和心跳清晰可见,肛突明显,背鳍原基出现;破膜后20d,全长为21.25±1.40mm,此时约半数卵黄囊体积明显减小,可以看见背鳍、臀鳍与尾鳍的出现;破膜后40d,个别发育较快的个体开始在水中游动,主动摄食;破膜后45d,卵黄囊基本吸收完全,仔鱼具有游泳能力,50d后几乎全部上浮。

    Abstract:

    Interspecific hybridization between Oncorhynchus mykiss (♀) and Oncorhynchus masou masou (♂) was conducted by artificial propagation.A series of normal stages based on morphological features for the embryonic and larvae development of the hybrid was described.The results showed that the total effective temperature of the embryonic development was 387.44 ℃·d which is higher than Oncorhynchus mykiss and lower than Oncorhynchus masou masou at the water temperature of 4.3-9.2 ℃, while the eyeball pigment stage kept on 206.01 ℃·d;the newly hatched larva was diaphanous all over the body and its blood circulation,heartthrob,anal stylet and dorsal fin rudiment could be seen clearly;20 days after hatching,half of the yolk sac of the larvae was absorbed,the total length was 21.25 ± 1.40 mm,and the dorsal fin,anal fin and tail fin could be seen;40 days after hatching,some of the larvae swam and took in food in the water;45 days after hatching,the yolk sac of the larvae that could swim was absorbed mostly;almost all of the larvae swam up 50 days after hatching.

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陈术强,张玉勇,贾智英,鲍宝龙,龚小玲.虹鳟(♀)×山女鳟(♂)杂交种胚胎及仔鱼发育的研究[J].上海海洋大学学报,2010,(6):756-762.
CHEN Shu-qiang, ZHANG Yu-yong, JIA Zhi-ying, BAO Bao-long, GONG Xiao-ling. A study on embryonic and larval development of hybrid F1 by Oncorhynchus mykiss (♀)× Oncorhynchus masou masou (♂)[J]. Journal of Shanghai Ocean University,2010,(6):756-762.

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  • 收稿日期:2010-05-19
  • 在线发布日期: 2022-11-22
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