Mass moralities of Aerossocheilus fasciatus with red-skin were occurred in Xinchang fishery farm, Zhejiang province, in March 2010.The bacteria strain GCL100301 was isolated from liver of moribund Aerossocheilus fasciatus and identified by the bacteriological and molecule biology method, which were identified as Pseudomonas fluorescens.The strain was sensitive to Ciprofloxacin and Neomycin and resistant to Penicillin, SMZ+TMP, Cefazolin, Novobiocin, Furazolidone and Ceftofur.It would provide theoretical event for prevention and cure this disease.