The growth of spoilage bacteria and the rate of production of metabolite for sterile Pseudosciaena crocea tissue blocks inoculated Shewanella putrefaciens and Pseudomonas spp. stored aerobically at 5 ℃ were respectively assayed. The yield factor for production of metabolite and the cell concentration determined at the time of off-odour detection were used as quantitative measurements of microbial spoilage ability. At spoilage point,the logarithm counts of the N_s (minimum number of spoilage bacteria) of Shewanella putrefaciens and Pseudomonas spp. were 9.14 lg cfu/g and 9.25 lg cfu/g,respectively. Adopting Gompertz regression equation to describe the growth curve of Shewanella putrefaciens and Pseudomonas spp.,we gained growth kinetics model parameters of both spoilage bacteria. The μ_max (maximum specific growth rate) of Shewanella putrefaciens and Pseudomonas spp. were 0.071 4 and 0.070 0,the logarithm counts of the N_max (maximum number of bacteria) of them were 9.45 lg cfu/g and 9.37 lg cfu/g,respectively. The results showed that their μ_max were basically the same and both of N_max were more than N_s of them,suggesting both spoilage organisms could be responsible for spoilage of Pseudosciaena crocea. The Y_TVBN/cfu and Y_TMA/cfu of Shewanella putrefaciens were 1.38×10^-10 mg-N TVBN/cfu and 1.31×10^-10 mg-N TMA/cfu,respectively. The Y_TVBN/cfu of Pseudomonas spp. was 1.18×10^-10 mg-N TVBN/cfu. The quantitative results indicated that the spoilage ability of both spoilage bacteria was equivalent. Because Shewanella putrefaciens and Pseudomonas spp. were the dominant spoilage bacteria when Pseudosciaena crocea was stored aerobically at low temperature,also based on the concept of SSO (Specific spoilage organism),Shewanella putrefaciens was the most dominant spoilage bacterium in the specific spoilage organisms,followed by Pseudomonas spp.