16S rDNA文库法分析草鱼肝胰脏和胆汁细菌群落组成
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S917

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上海市教委重点学科建设项目(J50701和S30701)


Analysis of the composition of bacterial communities in the hepatopancreas and bile of Ctenopharyngodon idellus through sequencing 16S rDNA library
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    摘要:

    利用16S rDNA文库随机测序法分析了草鱼肝胰脏和胆汁细菌菌落组成,结果表明,在肝胰脏中共鉴定出19种共生细菌,分别隶属于气单胞菌属、假单胞菌属、摩根菌属、不动杆菌属、弧菌属和变形杆菌属。气单胞菌属和假单胞菌属为优势属,气单胞菌属的丰度为36.17%,假单胞菌属为31.91%。其中,气单胞菌属的GZ16和假单胞菌属的GZ9菌株丰度最高,均为12.77%。在胆汁中共鉴定出10种共生细菌,分别隶属于气单胞菌属、假单胞菌属、摩根菌属和变形杆菌属。气单胞菌属和假单胞菌属为优势属,气单胞菌属的丰度为35%,假单胞菌属为27.5%。其中,变形杆菌属的DN1菌株丰度最高,为25%。肝胰脏中只有10.5%的细菌群落与胆汁的细菌群落相似,胆汁中只有20%的细菌群落与肝胰脏相似,表明草鱼肝胰脏和胆汁有各自特有的共生细菌群落。本研究结果,为进一步探讨肝胰脏和胆汁共生细菌的生理功能,以及细菌与宿主草鱼之间的协同进化提供了基础。

    Abstract:

    The bacterial diversity and community composition in hepatopancreas and bile of grass carp Ctenopharyngodon idellus were determined through seqencing the 16S rDNA gene library. The bacterial spectrum of hepatopancreas included eight species of Aeromonas,six species belonging to Pseudomonas,two species from Morganella,and one species each from Vibrio and Proteus. Aeromonas and Pseudomonas were two dominant genera with 36.17% and 31.91% relative abundance of community,respectively. Strains GZ16 of Aeromonas and GZ9 of Pseudomonas were two dominant species sharing same 12.77% relative abundance. The bacterial spectrum of bile included five species of Aeromonas,three species of Pseudomonas,and one species each from Morganella and Proteus. Same as found in hepatopancreas,Aeromonas and Pseudomonas were two dominant genera,sharing 35% and 27.5% relative abundance of community,respectively. Strain DN1 of Proteus was dominant species with 25% relative abundance of community. Comparative analysis showed that 10.5% community species in the hepatopancreas shared same as that in the bile,and 20% community species in the bile shared same as that in the hepatopancreas. This means there was a specific parasitic community in hepatopancreas or bile of grass carp. The results present in this research should be helpful to further understand the unknown roles of these bacteria in the hepatopancreas and bile,also to understand coevolution mechanism between bacterial community and host grass carp.

    参考文献
    [1] McFall-Ngai MJ,Henderson B,Ruby E G.The Influence of Cooperative Bacteria on Animal Host Biology[M].Cambridge:Cambridge University Press,2006.
    [2] Saha S,Roy R N,Sen S K,et al.Characterization of cellulase-producing bacteria from the digestive tract of tilapia,Oreochromis mossambica(Peters)and grass carp,Ctenopharyngodon idella(Valenciennes)[J].Aquacul Res,2006,37(4):380-388.
    [3] Rimmer D W,Wiebe W J.Fermentative microbial digestionin herbivorous fishes[J].Journal of Fish Biology,1987,31:229-236.
    [4] Clements K D,Sutton D C,Choat J H.Occurrence and cha-racteristics of unusual protistan symbiosis from surgeon fishes(Acanthuridae)of the Great Barrier Reef,Australia[J].Marine Biology,1989,102(3):403-412.
    [5] Clements K D.Endosymbiotic communities of two herbivorous labroid fishes,Odaxcyanomelas,andO.Pullus[J].Marine Biology,1991,109(2):223-229.
    [6] Luczkovich J J,Stellwag E J.Isolation of cellulolytic microbes from the intestinal tract of the pinfish,Lagodon rhomboids:size-related changes in diet and microbial abundance[J].Marine Biology,1993,116(3):381-388.
    [7] Bairagi A,Sarkar Ghosh K,Sen S K,et al.Enzyme pro-ducing bacterial flora isolated from fish digestive tracts[J].Aquaculture International,2002,10(2):109-121.
    [8] Hooper L V,Gordon J I.Commensal host-bacterial relation-ships in the gut[J].Science,2001,292(5519):1115-1118.
    [9] Guarner F,Malagelada J R.Gut flora in health and disease[J].Lancet,2003,361(9356):512-519.
    [10] Rawls J F,Samuel B S,Gordon J I.Gnotobiotic zebrafish reveal evolutionarily conserved responses to the gut microbiota[J].Proc Natl Acad Sci USA,2004,101(13):4596-4601.
    [11] 周文豪,陈孝煊,张冬晓,等.摄食不同饵料对草鱼肠道菌群影响的研究[J].华中农业大学学报,1998,17(3):252-255.
    [12] Lindsay G J H,Harris J E.Carboxymethylcellulase activity in the digestive tracts of fish[J].J Fish Biol,1980,16(3):219-233.
    [13] Lesel R,Fromageot C,Lesel M.Cellulose digestibility in grass carp,Ctenopharyngodon idellaand in goldfish,Carassius auratus[J].Aquaculture,1986,54(1-2):11-17.
    [14] Cahill MM.Bacterial flora of fishes:a review[J].Microbiol Ecol,1990,19(1):21-41.
    [15] Mcvicar A H.Interactions of pathogens in aquaculture with wild fish populations[J].Bull Eur Ass Fish Pathol,1997,17(6):197-200.
    [16] Nieto TP,Toranzo A E,Barja J L.Comparison between the bacterial flora associated with fingerling rainbowtrout cultured in two different hatcheries in the North-West of Spain[J].Aquaculture,1984,42(3-4):193-206.
    [17] De Sousa J A,Silva-Sousa AT.Bacterial community associated with fish and water from Congohas River,Sertaneja,Parana,Brasil[J].Brazilian Archives of Biology and Technology,2001,44(4):373-381.
    [18] Shakibazadeh S,Saad C R,Christianus A.et al.Bacteria flora associated with different body parts of hatchery reared juvenilePenaeus monodon,tanks water and sediment[J].Annals of Microbiology,2009,59(3):425-430.
    [19] 杨桂梅,唐文乔,鲍宝龙,等.利用PCR-DGGE法分析暗纹东方魨的弧菌菌落组成[J].上海水产大学学报,2006,15(3):257-263.
    [20] Yang G,Bao B,Peatman E,et al.Analysis of the composi-tion of the bacterial community in puffer fish.Takifugu obscurus[J].Aquaculture,2007,262(2-4):183-191.
    [21] Yang G,Bao B,Li H.et al.Analysis on the composition of culturable bacterial communities in several tissues of puffer fishTakifugu obscurus[J].Journal of Shanghai Fisheries University,2008,17(1):12-21.
    [22] 赵庆新,谭远德.利用肠道菌群的分布和DNA序列对鲤科鱼类系统演化关系的研究[J].2001,中国水产科学,8(1):26-32.
    [23] Bosshard P P,Santini Y,Grüter D,et al.Bacterial diversity and community composition in the chemocline of the meromictic alpine Lake Cadagno as reveal by16S rDNA analysis[J].FEMS Microbiol Ecol,2000,31(2):173-182.
    [24] Tamura K,Dudley J,Nei M.et al.MEGA4:Molecular evo-lutionary genetics analysis(MEGA)software version4.0[J].Mol Biol Evol,2007,24(8):1596-1599.
    [25] 李小勤,冷向军.鱼类胆汁的应用[J].天津农业科学,2006,12(4):22-25.
    [26] Li W,Scott A P,Siefkes M J,et al.Bile acid secreted by male sea lamprey that acts as a sex pheromone[J].Science,2002,296(5565):138-141.
    [27] 尾崎久雄.鱼类消化生理(上册)[M].吴尚忠,译.上海:科学技术出版社,1983.
    [28] Felske A,Akkermans AD L,De Vos WM.In situ detection of an uncultured predominant Bacillus in Dutch grassland soils[J].Appl Environ Microbiol,1998,64(11):4588-4590.
    [29] Fritsche TR,Horn M,Seyedirashti S,et al.In situ detection of novel bacterial endosymbionts of Acanthamoeba spp.Phylogenetically related to members of the order Richettsiales[J].Appl Environ Microbiol,1999,65(1):206-212.
    [30] Tanaka R,Ootsubo M,Sawabe T,et al.Biodiversity and in situ abundance of gut microflora of abalone(Haliotis discus hannai)determined by culture-independent techniques[J].Aquaculture,2004,241(1-4):453-463.
    [31] Li Z,Hu Y,Liu Y,et al.16S rDNA clone library-based bacterial phylogenetic diversity associated with three South China Sea sponges[J].World J Microbiol Biotechnol,2007,23(9):1265-1272.
    [32] Liebner S,Harder J,Wagner D.Bacterial diversity and community structure in polygonal tundra soils from Samoylov Island,Lena Delta,Siberia[J].Int Microbiol,2008,11(3):195-202.
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蒋燕,鲍宝龙,谢彩霞,杨桂梅.16S rDNA文库法分析草鱼肝胰脏和胆汁细菌群落组成[J].上海海洋大学学报,2010,(4):440-446.
JIANG Yan, BAO Bao-long, XIE Cai-xia, YANG Gui-mei. Analysis of the composition of bacterial communities in the hepatopancreas and bile of Ctenopharyngodon idellus through sequencing 16S rDNA library[J]. Journal of Shanghai Ocean University,2010,(4):440-446.

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  • 收稿日期:2010-02-18
  • 在线发布日期: 2022-11-21
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