According to the gene sequence of thermostable direct hemolysin in GenBank,thermostable direct hemolysin gene was subcloned into an Escheria coli expression vector pGEX-4T-1.Recombined plasmids tdhA/pGEX-4T-1,tdhS/pGEX-4T-1 were then transformed into Escheria coli BL21(DE3).The recombinant plasmid tdhA/pGEX-4T-1 and tdhS/pGEX-4T-1 had stable and efficient expression in BL21,the high pure fused protein could be obtained by using affinity chromatography method.The result of hemolytic test showed that the prot...