The color and yield of the tipids and phospholipids were better by enzymatic hydrolysis and ethanol extraction method, with PC as the main component in the extracted phospholipids. Method of. hexane and ethanol extraction and then washing by acetone could easily and efficiently extract total phospholipids, and the neutral lipids rich in sterols and another neutral lipids with lower sterols. Acetone washing could remove the neutral lipids from the phospholipids, especially the sterol and triterpenoic alcohols which are easily dissolved in the alcohol, and only a little poisonous organic solvent was used. Though the classic method Bligh-Dyer is handy, the large amount of noxious solvent it used made it unsafe. In addition, higher purity of PC and PE are prepared from the hexane and ethanol extracted phospholipids by low pressure column chromatography with methanol as washing solvent and SPD-10A detector.
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陆丽丽,陈舜胜.鲫鱼卵中脂质提取方法的比较[J].上海海洋大学学报,2008,(1):104-108. LU Lili, CHEN Shun-sheng. Comparison on methods of extracting lipids from roes of Carassius auratus[J]. Journal of Shanghai Ocean University,2008,(1):104-108.