Divergent primers were designed for HSP amplification according to the sequence in Genbank(EMB: AF506290).After PCR and sequencing,it was showed that the HSP gene sequence of P.crocea was about 1.7 kb in length.According to the phylogenic tree,the tree consisted of some distinct branches,which were constructed by HSP of different species,and animals belonged to the same branch.And P.crocea shared highest homology with Venous leaves(African clawed frog),which confirmed the hypothesis that the Amphibian was subsequent to the Pisces in the evolutionary lineage partially.And the construction work in this paper could lay a basis for subsequent work of nucleotide probe preparation,clone and selection of some kinds of fine functional genes for culture,construction of P.crocea cDNA library,and was of great meaning for the development for the culture of P.crocea.