A function-checking vector,pYF3553 was constructed,GUS gene as the reporter gene,whose upstream was the fusion-promoter consisted of cis-acting elements of Lentinula edodes and a yeast Cyc1 gene mini-promoter.Two function-checking vector,whose cis-acting elements of Lentinula edodes were XG108 and XG111,were named pXG108 and pXG111.By transforming of the pXG108 into protoplasts of Lentinula edodes,transient expression of GUS activity was twice as high as the control.This phenomenon revealed the obvious expression of GUS gene and the modulation function of its upstream sequence.Transient GUS gene expression of the two vector,pXG108 and pXG111,was different.Difference existed in the transient GUS gene expression between the two cis-acting elements of Lentinula edodes,pXG108 and pXG111 resemble the expression effect in yeast of that.A detailed analysis of superiority and disadvantage for the method utilizing the transient GUS gene expression to detect the unknow function of cis-acting elements of edible fungi was also brought forward.