Haemocyte subpopulation percentage variety of Chlamys farreri was studied by light-scatter flow cytometry,and the aim is to determine the role of zymosan and glycine-Zn in the immune response of molluscs.After injection of zymosan and glycine-Zn,the subpopulation percentages variety of the haemocytes were assayed at 6 h,12 h,24 h,48 h,96 h and 144 h.Three haemocyte groups,hyalinocyte,small granulocyte and large granulocyte,were found in the haemolymph of Chlamys farreri by the analysis method of two parameter plots(FSC,Forward Scatter and SSC,Side Scatter).After injection with zymosan,the hyalinocyte percentage in haemocytes of experimental groups were significantly higher than that of control groups at 6 h,12 h,24 h and 48 h.But the small granulocyte percentage were significantly lower than that of control at 6 h,12 h and 24 h.After injection with glycine-Zn,the hyalinocyte percentage in haemocytes was significantly higher than that of control groups at 12 h,24 h and 48 h.However,small granulocyte percentage was significantly lower than that of control groups at 12 h,24 h and 48 h.These results indicated that zymosan and glycine-Zn had great effects on haemocyte subpopulation percentages variety of Chlamys farreri,and they could cause the increase of hyalinocyte and decrease of granulocyte.