Plasmid pYH2555 was generated by exciting pBSK II with EcoR I and Hind III and inserting the promoter of gpd,EGFP gene and CaMV 35S terminator sequence from plasmid pBGgHg.Intermediate plasmid pYH2873 was made by digesting pCAMBIA1300 with EcoR I and Hind III and inserting the promoter of gpd,EGFP gene and CaMV 35S terminator sequence from pYH2555.Then pYH2873 was digested by Hind III and ligated to the trp3iar gene which was excited from plasmid pDB06.The plasmid pYH2873 was digested by EcoR I and Hind III, and electrophoresis of the digested products showed two fragments: 1.3 bp fragment and 9 kb fragment.The expression vector pSAGF was digested by Hind III,and electrophoresis of the digested products showed two fragments: 4 kb fragment and 10 kb fragment.Sequencing analysis showed that the recombinant plasmid was correct.Thus the pSAGF,a binary expression vector of V. volvacea,was constructed successfully.