To test some presumed systematic classifications of sinipercine fishes,16S rDNA gene of some sinipercine fishes were PCR amplified and sequenced,a preliminary phylogenetic relationship of sinipercine fishes and some lower percoids was constructed using the homogenous sequences of 16S rDNA.The results showed sinipercine fishes was a monophyletic group,however,it didn't cluster with Serranidae and didn't show close relationships with Percichthyidae,Moronidae,Centropomii and Lateolabrax.Compared with the studied percoids, sinipercine fishes is a specialized freshwater group and lies at the top of the tree,which may support its rise as an independent family Sinipercidae.Our results may serve as a molecular base for further understanding the phylogeny of lower percoids.