Penaeus chinensis juvenile were fed on basal diets supplemented with different levels Vc (0,0.015%,(0.030%),0.045%,0.060%), beta-glucan (0,0.050%,0.075%,0.100% )and algae powder(0,2%,5%,7% )for 40 days respectively.The shrimp fed on basal diet were regarded as control . Each group was treated with triplicate. Growth, survival rate and activities of acid phosphates(ACP), catalase (CAT), superoxidase(SOD) and lysozyme(LSZ) were measured.The relative body length gain and CAT activity of 0.030% Vc group were significantly higher than those of the control(P<0.05). The activities of ACP and CAT of 0.045% Vc group were enhanced(P<0.05). The activities of SOD were not affected by Vc supplement(P<0.05).The LSZ activities of 0.015% Vc group were significantly increased (P<0.01). The relative body length gain and SOD activity of (0.075%) beta-glucan group were significantly higher than those of the control(P<0.05). The relative body length and weight gain of 7% algae powder group were significantly increased(P<0.01),but the activity of ACP and LSZ of the group was decreased (P<0.05). The activities of CAT of 5% algae powder were enhanced(P<0.05).