Using two-factor, five-level, orthogonal regression rotational combination design, using fishmeal as animal protein and soybean meal as plant protein, to formulate 9 diet combinations, 850 Pelteobagrus fulvidraco with an average intial weight of 11.4g, were randomly divided into 17 groups (one control and 16 treatments) in feeding trial for 68d to study the optimal levels of crude protein in diets and the optimal percentage of animal protein in crude protein. The results indicated that the optimal levels of crude protein in diets were 42.5% and the optimal percentage of animal protein in crude protein was 67%. They produced fastest growth, highest daily gain and constitute best ratio in the diet.