Total RNA was isolated from Cirrhinus molitorella liver tissue. The cDNA encoding insulin like growth factor I (IGF I) was amplified by reverse transcription polymerase chain reaction (RT PCR) strategy using isolated total RNA as template.The amplified cDNA fragment was inserted to vector pUCm T. The cloned cDNA was sequenced and the amino acid sequence of Cirrhinus molitorella IGF I was predicted .The nucleotide sequence and deduced amino acid sequence shared 95.2% and 100% homology with that of Cyprinus carpio mature IGF I peptide. Analysis of E domain indicated that cloned Cirrhinus molitorella belonged to IGF I Ea 2 subtype.