At three experimental stations, Qingdao, Guangzhou and Bengbu, based on GIFT strain of Nile tilapia, Oreochromis niloticus and mass selection method, F 3 was produced and evaluated. In three experimental stations, the daily growth rate of F 3 was 14.1%, 7.5% and 80.8% higher than that of control line respectively, the average selected response per generation was 4.7%, 2.5% and 26.9% respectively. In selected line, morphology traits, such as strips on body side and caudal fin were more clear and typical than control line, and the variation coefficient of body weight was reduced. These indicated that the selected line has been more purified. The value of growth rate and selection respones from Bengbu station were much higher than those of other two experimental stations, it could relate to the more alternation of experimental environment in Bengbu, which indicated that a high attention to gene environment interaction should be paid in further selection study.