Random amplified polymorphic DNA(RAPD) markers were used to detected 4 populations genetic variation of 20 Chinese shrimps (Penaeus chinensis) along the coast of China, 5 from each of samples caught off Yantai, Changdao, Qingdao, and also for 5 individuals from a cultured sample collected off Ningbo. 9 primers screen from 50 random primers generated 54 RAPD bands, among which 36 were varied. The intra population genetic distances among those 4 populations (Yantai, Changdao, Qingdao, Ningbo) were 0.0154,0.0370,0.0462,0.0550, respectively. The inter population genetic distances among them were 0.0793,0.1139,0.0988,0.1485,0.0880,0.1260, respectively. Molecular phylogenetic tree constructed by UPGMA and NJ method suggested that there were some genetic variation among different geographic population.