Abstract:The genetic assessment of 64 wild and cultivated mud carp, Cirrhina molitorella, 10 India mrigal, C. mrigola and 10 India rohu, Label rohita is inferred from its RAPD analysis. 107, 73, 87 DNA fragments in mud carp, India mrigal and India rohu are amplified respectively by 26 10bp-primers. The polymorphic loci percentage is 35. 5%,39. 7%, 37. 9% respectively. The genetic distance of mud carp-India rohu, mud carp-India mrigal and India mrigal-India rohu is 0. 22, 0. 58, 0. 64 respectively. The relationship of mud carp-India rohu is more close than mud carp-India mrigal and India mrigal-India rohu by the dendrogram of the three species based on UPGMA and NJ. This is similar to allozyme analysis, but there is differential from traditional taxonomy.