This paper describes that GCHV-892 has been domesticated continuously for 19 generations in cell 1ine (PSF) in culture solution with certain concentration of liquid being from An (Eucalyptus) leaves, and has been attenuated. Effect of attenuation has been stabilized for continuous domestication from 19 to 29 gen. The effect of attenuation has still been stabilized for continuous domestication up to 37 gen. After the An liquid did not add to the culture solution from 27~37 gen., there are good innocuity and strong immunogenicity of virus for grass carp. Attenuated live vaccine prepared with the attenuated strain for grass carp is 100% in innocuity survival rate and immune protection rate. It shows that the An liquid is a good attenuated medicine for virus of hemorrhage of grass carp, and GCHAV-892 attenuated with An liquid is a good attenuated strain.