Vibrio parahaemolyticus hemoD knockouted and pathogenicity evaluation
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S917

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    Abstract:

    Vibrio parahaemolyticus is a Gram-negative rod-shaped bacterium with flagella, widely distributed in estuaries and oceans, and is one of the major pathogenic bacteria in aquaculture. For future development of attenuated vaccine of V. parahaemolyticus, in this study we knocked out hemoD gene in V. parahaemolyticus using homologous recombination. Compared with wild type of V. parahaemolyticus, ΔhemoD strain showed a decrease in hemolytic activity by 45.7%, a decrease in the ability of biofilm formation by 36.2%, and no significant change in growth rate. When co-cultured with Tetrahymena thermophila, the injury of Tetrahymena by ΔhemoD strain was less with more Tetrahymena survived, and hence engulfed more cells of ΔhemoD strain than the wild strain of V. parahaemolyticus. The whiteleg shrimp Penaeus vannamei was further used for pathogenicity evaluation. The P. vannamei werechallenged with strain ΔhemoD and wild type strain of V. anguillarum through administration, respectively, the LD50 of ΔhemoD strain was 5.06 times higher than that of the wild type strain. Compared with wild type of V. parahaemolyticus, the colonization ability of ΔhemoD strain in the intestinal tract of P. vannamei was obviously weakened, and the damage ability to hepatopancreas was reduced, and the phenomena of hepatopancreatic cytolysis and vacuolisation were less serious. These results indicate that the ΔhemoD strain has a reduced pathogenicity to P. vannamei. Onthe whole, we constructed an attenuated strain of V. parahaemolyticus, which will be developed as a potential attenuated vaccine.

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胡少杰,车金远,房启童,罗土炎,鲍宝龙.副溶血弧菌hemoD敲除菌株构建及致病力评估[J].上海海洋大学学报,2025,34(3):686-695.
HU Shaojie, CHE Jinyuan, FANG Qitong, LUO Tuyan, BAO Baolong.Vibrio parahaemolyticus hemoD knockouted and pathogenicity evaluation[J]. Journal of Shanghai Ocean University,2025,34(3):686-695.

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History
  • Received:May 27,2024
  • Revised:June 28,2024
  • Online: May 23,2025
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