文章摘要
鳜幽门盲囊早期发育的组织学特征
Histological features of organogenesis of the pyloric caecum of Siniperca chuatsi
投稿时间:2019-11-06  修订日期:2020-06-05
DOI:
中文关键词:   幽门盲囊  器官发生  组织学
英文关键词: Siniperca chuatsi  Pyloric caecum  Organogenesis  Histology
基金项目:现代农业产业技术体系专项(No.CARS-46);
作者单位邮编
赵亮亮 上海海洋大学 201306
施琳澜 上海海洋大学 201306
赵金良 上海海洋大学 201306
周云红 上海海洋大学 201306
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中文摘要:
      采用组织切片、H.E和AB.PAS染色技术对不同日龄鳜的幽门盲囊数量、组织学特征以及粘液细胞类型和分布进行了观察和描述。结果表明:15日龄鳜在胃肠之间左腹侧有一枝,幽门盲囊开始发育;20日龄鳜在肠下方右腹侧出现一簇幽门盲囊,25日龄鳜在胃肠之间右腹侧和肠下方左腹侧各出现一簇幽门盲囊;随着日龄增加,鳜的幽门盲囊数量继续增加。20、30、40、50和60日龄,鳜的幽门盲囊数量分别为25±2、75±2、123±4、155±7、190±5条。幽门盲囊的长度、肌层厚度及内侧的皱襞数量,也随鳜发育日龄而呈现递增趋势。幽门盲囊由多枝汇集成簇,每枝在中部产生2个分支,盲囊顶部比基部皱襞数量少且长度短。幽门盲囊粘液细胞以Ⅱ型为主,Ⅳ型次之,Ⅲ型最少,未见Ⅰ型粘液细胞,盲囊基部粘液细胞数量显著多于顶部。
英文摘要:
      The number, histological characteristics, types and distribution of mucus cells in the pyloric caecum of Siniperca chuatsi at different developmental age were observed and described by histology sectioning, H.E and AB. PAS stainings. The results showed that at 15 days after hatching, there was a cluster of pyloric caecum began to develop on the left ventral side between the stomach and intestinal. At 20 days after hatching, there was a cluster of pyloric caecum developed on the right ventral side below the intestinal tract, and at 25 days after hatching, there was a cluster of pyloric caecum developed on the right ventral side between the stomach and intestinal side, and the left ventral side below the intestinal, respectively. At the age of 20, 30, 40, 50 and 60 days, the number of pyloric caecum were 25±2, 75±2, 123±4, 155±7 and 190±5, respectively. The number and the length of the fold and the thickness of the muscular layer of pyloric caecum increased with development age. The pyloric caecum is clustered by multiple branches, each of which produces two sub-branches in the middle section. Ⅱ type of mucous cell was widely distributed in the pyloric caecum, followed by type Ⅳ, there was not Ⅰ type mucous cell. The amount of mucous cells on the base pyloric caecum were higher than the top part.
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