文章摘要
赵金涛,江敏,王俊南.超声-纤维素酶法提取狐尾藻抑藻成分的工艺优化[J].上海海洋大学学报,2015,24(4):560-569
超声-纤维素酶法提取狐尾藻抑藻成分的工艺优化
Optimal extraction of inhibitory components from Myriophyllum spicatum with the ultrasonic-cellulase method
投稿时间:2015-04-13  修订日期:2015-05-19
DOI:
中文关键词: 超声波-纤维素酶法  狐尾藻  铜绿微囊藻  生长阻碍率
英文关键词: ultrasound-cellulase method  Myriophyllum spicatum  M. aeruginosa  growth inhibition rate
基金项目:上海市教委科研创新项目(10ZZ103);上海市教委重点学科建设项目(J50701);上海市高校知识服务平台项目(ZF1206)
作者单位E-mail
赵金涛 上海海洋大学 水产与生命学院, 上海 201306  
江敏 上海海洋大学 水产与生命学院, 上海 201306
上海海洋大学 上海市水域环境生态上海高校工程研究中心, 上海 201306 
mjiang@shou.edu.cn 
王俊南 上海海洋大学 水产与生命学院, 上海 201306  
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中文摘要:
      研究了超声波-纤维素酶法和水浸提法提取狐尾藻抑藻成分对铜绿微囊藻的抑制作用。以提取液对铜绿微囊藻的生长阻碍率为评价指标, 通过单因素和正交试验获得超声波-纤维素酶法提取狐尾藻化感物质的最优工艺条件:酶解时间为4 h, 最适pH为3.0, 温度为30 ℃, 酶用量(纤维素酶质量/藻粉质量)为21%, 超声波功率为120 W, 超声时间为1 h。超声波-纤维素酶法所得狐尾藻提取液对藻细胞的生长阻碍率远远高于水浸提法, 两者存在显著性差异(P<0.05)。随着反应时间延长, 超声波-纤维素酶提取液对铜绿微囊藻的生长阻碍率持续降低, 在提取液浓度为40 g/L, 反应时间为24 h时, 其对铜绿微囊藻的生长阻碍率为271.74%, 反应时间为13 d时, 对铜绿微囊藻的生长阻碍率仍达20%以上, 显著高于其他组别(P<0.05)。
英文摘要:
      The article explained that inhibitory components from Myriophyllum spicatum with the ultrasonic-cellulase method and water extraction method caused inhibition of M.aeruginosa. Based on one-way and orthogonal test, and regarding growth inhibition rate of extraction on M. aeruginosa as index of evaluation, an optimal extraction process of Myriophyllum spicatum was obtained as follows: enzymatic hydrolysis time was 4 h, pH was 3.0, optimal temperature was 30 ℃, cellulase dosage(mass of cellulase to Myriophyllum spicatum powder) was 21%, ultrasonic power was 120 W and ultrasonic time was 1h. The inhibition rate of extraction obtained with water extraction method and ultrasonic-cellulase method indicated that the latter showed better effect and had obvious difference with the water extraction (P<0.05). With the increasing of reaction time, the growth inhibition rate of the ultrasonic-cellulase extraction on M. aeruginosa continued to decrease, which was 271.74% at concentration of 40 g/L and reaction time for 24 h and the inhibition rate was more than 20% by the reaction time for 13d with the obvious difference with the other concentration groups (P<0.05).
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