文章摘要
孙世玉,江敏,金若晨,戴习林,吴昊,周俊芳,于忠利,张峰.凡纳滨对虾池塘养殖过程中水质与虾虹彩病毒病发生的相关性[J].上海海洋大学学报,2020,29(5):641-649
凡纳滨对虾池塘养殖过程中水质与虾虹彩病毒病发生的相关性
Correlation between water quality and shrimp hemocyte iridescent virus disease occurrence of Litopenaeus vannamei in ponds
投稿时间:2020-03-07  修订日期:2020-06-08
DOI:10.12024/jsou.20200302951
中文关键词: 凡纳滨对虾  虾虹彩病毒  水温  主成分分析法  正交偏最小二乘法
英文关键词: Litopenaeus vannamei  shrimp hemocyte iridescent virus  water temperature  principal component analysis  orthogonal projections to latent structures discrimination analysis
基金项目:虾类绿色配合饲料的研发与应用(沪农科创字〔2019〕第3-5号);上海市产业技术体系建设项目(沪农科产字〔2014〕第5号);上海市高校知识服务平台项目(ZF1206)
作者单位E-mail
孙世玉 上海海洋大学 海洋生态与环境学院, 上海 201306  
江敏 上海海洋大学 海洋生态与环境学院, 上海 201306
上海市水产养殖工程技术研究中心, 上海 201306 
mjiang@shou.edu.cn 
金若晨 上海海洋大学 海洋生态与环境学院, 上海 201306  
戴习林 上海市水产养殖工程技术研究中心, 上海 201306
上海海洋大学 水产与生命学院, 上海 201306 
 
吴昊 上海市水产养殖工程技术研究中心, 上海 201306
上海海洋大学 水产与生命学院, 上海 201306 
 
周俊芳 中国水产科学研究院东海水产研究所, 上海 200090  
于忠利 上海市奉贤区水产技术推广站, 上海 201419  
张峰 上海思阳水产养殖合作社, 上海 201400  
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中文摘要:
      为了解凡纳滨对虾(Litopenaeus vannamei)养殖过程中水质与病害发生之间的关联性。对上海市奉贤区某虾类养殖合作社的池塘水质、养殖虾病原携带情况进行定期检测,结合其发病状况,使用主成分分析法(PCA)和正交偏最小二乘法(OPLS-DA),分析健康组、带病组、发病组同一时间段水质数据的差异性,并筛选出对各组差异贡献最大的水质因子。试验中,带病组和发病组的养殖虾均有检出虾虹彩病毒,分析表明:(1)发病组与健康组之间、发病组与带病组之间,水质有显著差异;而只要不发病,即便养殖虾携带病毒,该池塘水质与健康组之间并无显著区分。(2)水质因子与虾病发生的关联性程度为总氮 > 水温 > 总磷 > 溶解氧。(3)分塘等机械操作在一定程度上也会引起凡纳滨对虾的应激反应,诱发虾病的发生。(4)“带病&健康”组为大棚养殖,水温保持在30℃左右,即使凡纳滨对虾带有虾虹彩病毒,依然可以健康生长,但将其转移至较低水温条件下的露天池塘继续养殖时,则疾病暴发,出现死亡。养殖过程中应重视水温管理,注意水质变化,减少不必要的机械操作活动。
英文摘要:
      Water quality analysis and virus detection was carried out in a shrimp breeding cooperative in Fengxian District, Shanghai in order to understand the correlation between water quality and diseases occurrence of Litopenaeus vannamei. Combined with situation of disease occurrence, principal component analysis (PCA) and orthogonal projections to latent structures discrimination analysis (OPLS-DA) were used to figure out the difference of water quality with in same period among healthy group, virus carrying group and diseased group, and find those water quality factors that contributed most to the differences among three kinds of groups.In this research, shrimp hemocyte iridescent virus was detected in some shrimp samples and the results showed that: (1) Obvious water quality difference existed between diseased group and healthy group, also between diseased group and virus carrying group. While no obvious difference was found between healthy group and virus carrying group without disease out breaking. (2)The correlation degreefrom high to low between water quality factors and the occurrence of shrimp disease is total nitrogen > water temperature > total phosphorus > dissolved oxygen.(3) Mechanical operations such as diversion might cause stress response of shrimp and then induce disease. (4) Shrimp of “virus carrying & healthy group” was cultured in green house and water temperature was kept around 30 ℃, even if shrimp hemocyte iridescent virus was found, cultured shrimpcould grow normally; But when shrimp was transferred to lower temperature ponds, disease broke out.So water quality including temperature, etc., should be paid attention during the breeding of Litopenaeus vannamei and unnecessary mechanical operation activities should be avoided.
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