文章摘要
纪鹏,王伟隆,黄旭雄.不同C/N对绿球藻生长和细胞组分的影响[J].上海海洋大学学报,2020,29(4):542-551
不同C/N对绿球藻生长和细胞组分的影响
Effects of different C/N ratios on growth and cell biochemical components of Chlorococcum sp.
投稿时间:2019-08-25  修订日期:2019-12-12
DOI:10.12024/jsou.20190802775
中文关键词: 绿球藻  C/N  生长  生物量  细胞组分  脂肪酸
英文关键词: Chlorococcum sp.  C/N  growth  biomass  cell biochemical component  fatty acid
基金项目:上海市科技兴农项目(沪农科推字﹝2013﹞第2-1号);上海市科技兴农项目(沪农科攻字﹝2015﹞第1-2号)
作者单位E-mail
纪鹏 上海海洋大学 农业农村部淡水水产种质资源重点实验室, 上海 201306  
王伟隆 上海海洋大学 农业农村部淡水水产种质资源重点实验室, 上海 201306  
黄旭雄 上海海洋大学 农业农村部淡水水产种质资源重点实验室, 上海 201306
上海海洋大学 上海市水产养殖工程技术研究中心, 上海 201306
上海海洋大学 水产科学国家级实验教学示范中心, 上海 201306 
xxhuang@shou.edu.cn 
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中文摘要:
      研究了不同C/N(0:1、3:1、6:1、9:1、12:1和15:1)对绿球藻(Chlorococcum sp.)生长和细胞组分的影响,初始接种密度为300×104个/mL,实验进行7 d。结果表明:兼养培养能够显著提高绿球藻细胞密度、特定生长率和生物量(P<0.05),当C/N为9:1时,生物质量浓度达到最大值0.36 g/L;兼养条件下,随着C/N的增加,绿球藻蛋白质和总脂质量分数有所降低,C/N为12:1时,蛋白质质量分数达到最小值33.76%;C/N为15:1时,总脂质量分数达到最小值6.67%,显著低于其他各组(P<0.05);碳水化合物质量分数表现为先增加后降低的趋势,在C/N为9:1时达到最大值30.57%,显著高于其他各组(P<0.05);不同C/N对绿球藻脂肪酸组成和质量分数影响不同,随着C/N的增加,饱和脂肪酸(SFA)和单不饱和脂肪酸(MUFA)质量分数提高,在C/N为15:1时,分别达到最大值20.72%和14.61%,多不饱和脂肪酸(PUFA)质量分数降低,在C/N为15:1时,达到最小值64.67%。由此可知,C/N为9:1可以提高绿球藻生物量,随着C/N的增大有利于SFA和MUFA的合成,但不利于PUFA的合成。
英文摘要:
      To investigate the effects of different C/N ratios on the growth and cell biochemical components of Chlorococcum sp. Chlorococcum sp. was cultured with C/N ratios of 0:1, 3:1, 6:1, 9:1, 12:1 and 15:1 for 7 days, and the initial inoculation density was 300×104 cells/mL. The results showed that mixotrophic cultivation could significantly increase cell density, specific growth rate and biomass (P<0.05). While the ratio of C/N was 9:1, the biomass reached the maximum value of 0.36 g/L. Under mixotrophic cultivation, the protein and total lipid mass fraction of Chlorococcum sp. decreased with the increasing of C/N. When C/N was 12:1 and 15:1, the protein and total lipid mass fraction reached the minimum values of 33.76% and 6.67%, respectively. Carbohydrate mass fraction increased firstly and then decreased with the increasing of C/N. Furthermore, the maximum value (30.57%) was obtained by 9:1 group which was significantly higher than other groups (P<0.05). The mass fraction of saturated fatty acid (SFA) and monounsaturated fatty acid (MUFA) increased with the increasing of C/N ratios with the maximum values in 15:1 group. By contrast, increasing of C/N ratios had an adverse effect on polyunsaturated fatty acid (PUFA) content of Chlorococcum sp. with the minimum value (64.67%) in 15:1 group. It is concluded that the moderate up-regulation of C/N ratios could increase the biomass and promote the synthesis of SFA and MUFA of Chlorococcum sp., but not for PUFA.
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