文章摘要
许愈,张昭寰,赵莉,刘海泉,潘迎捷,赵勇.应用酸性电解水联合超声波杀灭副溶血性弧菌[J].上海海洋大学学报,2020,29(4):578-584
应用酸性电解水联合超声波杀灭副溶血性弧菌
Combination of acidic electrolyzed water with ultrasonic for killing Vibrio parahaemolyticus
投稿时间:2019-04-15  修订日期:2019-05-19
DOI:10.12024/jsou.20190402604
中文关键词: 副溶血性弧菌  酸性电解水  超声波  杀菌效果
英文关键词: Vibrio parahaemolyticus  acidic electrolyzed water  ultrasound  bactericidal effect
基金项目:国家自然科学基金(31671779,31571917);国家重点研发计划(2018YFC160220,2018YFC1602205);上海市科技兴农项目(沪农科攻字〔2016〕第1-1号,沪农科推字〔2017〕第4-4号);上海市教育委员会科研创新计划(2017-01-07-00-10-E00056);上海市教委曙光计划(15SG48)
作者单位E-mail
许愈 上海海洋大学 食品学院, 上海 201306  
张昭寰 上海海洋大学 食品学院, 上海 201306  
赵莉 上海海洋大学 食品学院, 上海 201306  
刘海泉 上海海洋大学 食品学院, 上海 201306
上海水产品加工及贮藏工程技术研究中心, 上海 201306
农业农村部水产品贮藏保鲜质量安全风险评估实验室, 上海 201306
上海海洋大学 食品热加工工程技术研究中心, 上海 201306 
 
潘迎捷 上海海洋大学 食品学院, 上海 201306
上海水产品加工及贮藏工程技术研究中心, 上海 201306
农业农村部水产品贮藏保鲜质量安全风险评估实验室, 上海 201306 
 
赵勇 上海海洋大学 食品学院, 上海 201306
上海水产品加工及贮藏工程技术研究中心, 上海 201306
农业农村部水产品贮藏保鲜质量安全风险评估实验室, 上海 201306 
yzhao@shou.edu.cn 
摘要点击次数: 96
全文下载次数: 76
中文摘要:
      将酸性电解水与超声波技术相结合,探究其对副溶血性弧菌的杀灭情况,并与其他杀菌措施进行对比。采用平板计数法进行菌落计数,扫描电镜观察细菌的形态变化,蛋白质泄漏揭示细胞膜通透性差异,并结合流式细胞仪分析生物学特征的改变,分别比较了酸性电解水、超声波以及联合处理对副溶血性弧菌的杀菌效果。结果显示:酸性电解水联合超声波处理可使副溶血性弧菌的数量减少2.09 log CFU/mL,亚致死菌数量为1.80 log CFU/mL,而仅用超声波处理,细菌只减少了0.63 log CFU/mL,亚致死菌数量为0.05 log CFU/mL(P<0.05)。扫描电镜结果表明,电解水联合超声波处理对副溶血性弧菌的细胞结构有明显的破坏作用,结合二喹啉甲酸法(BCA)显示其细胞内蛋白质泄漏226.596 μg/mL(P<0.05)。进一步的流式细胞仪分析结果显示,经联合处理后细菌细胞明显缩小,颗粒度变化增大。综上所述,相较于酸性电解水或超声波单一处理,通过菌落计数,细菌的形态变化,蛋白质泄漏与细胞生物学特征的改变可知,酸性电解水联合超声波的处理方式具有更强的杀菌效果,可作为一种新型的技术应用于水产品中副溶血性弧菌的杀灭。
英文摘要:
      This study evaluated the bactericidal effects were evaluated against Vibrio parahaemolyticus by acidic electrolyzed water (AEW) combined with ultrasound which is a method different from the traditional sterilization methods. The effects were compared by viable plate count analysis. The physiological response was studied by scanning electronic microscopy (SEM) and cell membrane permeability revealed by protein leakage. Flow cytometry analysis revealed changes in the physiological states of V.parahaemolyticus. The effects of the combination of SAEW and ultrasound on the reductions of V.parahaemolyticus were evaluated in comparison with acidic electrolyzed water or ultrasound. The results revealed that AEW combined with ultrasonic showed bacterial reduction in V.parahaemolyticus by 2.09 log CFU/mL and the sub-lethal bacterial reduction was 1.80 log CFU/mL,when compared to the reduction of 0.63 log CFU/mL and the sub-lethal bacterial reduction of 0.05 log CFU/mL(P<0.05) after ultrasonic treatment only. Scanning electron microscopy (SEM) results showed that electrolyzed water combined with ultrasonic treatment had obvious destructive effect on the cell structure of V.parahaemolyticus. The protein leakage of V.parahaemolyticus detected by bicinchoninic acid (BCA) were 226.596 μg/mL, respectively, and difference was significant (P<0.05). Flow cytometry analysis showed that the cells became smaller and the change in particle size increased. In summary, acidic electrolyzed water combined with ultrasound could be more effective in reducing V.parahaemolyticus, the physiological response changes, protein leakage and changes in the physiological states, when compared with acidic electrolyzed water or ultrasound alone. The combination of acidic electrolyzed water and ultrasound could be an new technique to reduce the risk of V.parahaemolyticus.
HTML 查看全文     下载PDF阅读器
关闭