云斑裸颊虾虎鱼体内各组织河鲀毒素的含量
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上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室,上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室,上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室,上海海洋大学 省部共建水产种质资源发掘与利用教育部重点实验室

基金项目:

国家自然科学基金(41176108);上海市教育委员会创新重点项目(14zz145)


Analysis of the tetrodotoxin content of tissues in Yongeichthys criniger
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The Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of Education,Shanghai Ocean University,The Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of Education,Shanghai Ocean University,The Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of Education,Shanghai Ocean University,The Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of Education,Shanghai Ocean University

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    摘要:

    我国曾发生多起食用虾虎鱼的中毒事件。从发生中毒事件的广东湛江徐闻县西连镇采集了有毒虾虎鱼,经形态学鉴定为云斑裸颊虾虎鱼(Yongeichthys criniger),其体内含河鲀毒素。采用酶联免疫法测定了云斑裸颊虾虎鱼各组织河鲀毒素(tetrodotoxin,TTX)的含量, 结果显示每千克组织的TTX含量由高到低依次为肝脏(69.954 mg)、卵巢(9.510 mg)、皮肤(8.937 mg)、肌肉(5.181 mg)、眼睛(3.321 mg)。比野生暗纹东方鲀(Takifugu fasciatus)相应组织TTX含量均要高,毒性强烈。

    Abstract:

    Food poisoning incidents have occurred in China because of eating goby, which is a species of fish living with a common edible mudskipper (Boleophthalmus pectinirostris).In order to investigate the goby species and its toxicity in this study, the goby samples were collected from Xuwen (Zhanjiang, Guangdong province) where the poisoning accident occurred in 2007.Based on the morphological features, the fish was identified as Yongeichthys criniger, which was reported as a tetrodotoxin (TTX)-accumulated fish species in Japan, and Taiwan province of China.The amount of TTX in each tissue of Y.criniger and Takifugu fasciatus was measured using enzyme linked immunosorbent assay.The tissue with TTX content in per kilogram tissue in Y.criniger from high to low is liver (69.954 mg), ovary (9.510 mg), skin (8.937 mg), muscle (5.181 mg), and eye (3.321 mg).The amount of TTX in each tissue of Y.criniger is higher than that in same tissue of Takifugu fasciatus.This is the first report about the amount of TTX in Y.criniger from mainland in China, and the investigation is helpful to prevent people eating toxic Y.criniger.

    参考文献
    [1] 伍汉霖, 钟俊生. 中国动物志: 硬骨鱼纲. 鲈形目. 虾虎鱼亚目[M]. 北京:科学出版社, 2008.
    [2] 杨霞. 因进食有毒海鱼,湛江16人中毒1人死亡[EB/OL]. http://www.gd.xinhuanet.com/newscenter/2007-03/13/content_9490844.htm,2007-03-13.
    [3] 陈杨,粤食安.毒倒22人,肇事的并非“跳跳鱼”经初步判断,祸起云斑裸颊虾虎鱼[N]. 新快报,2013-03-20 (A32).
    [4] NOGUCHI T, HASHIMOTO Y. Isolation of tetrodotoxin from a goby Gobius criniger [J]. Toxicon, 1973, 11(3): 305-307.
    [5] HASHIMOTO Y, NOGUCHI T. Occurrence of a tetrodotoxin-like substance in a goby Gobius criniger [J]. Toxicon, 1971, 9(1): 79-84.
    [6] HASHIMOTO Y, SHIOMI K. Occurrence of a skin toxin in coral-gobies Gobiodon spp[J]. Toxicon, 1974, 12(5): 523-524.
    [7] LIN S J, HWANG D F, SHAO K T, et al. Toxicity of Taiwanese gobies[J]. Fisheries Science, 2000, 66(3): 547-552.
    [8] 段发淼, 谢心磊, 朱宝平. 用小鼠单位法检测河豚毒素[J]. 中国卫生检验杂志, 2000, 8(4): 463-464.
    [9] 计融, 王健伟, 罗雪云, 等. 鲀毒鱼类中河鲀毒素直接竞争抑制性酶联免疫吸附试验测定方法的研究[J]. 中国食品卫生杂志, 2002,14(5):7-10.
    [10] 赖少阳, 张风雷, 岳亚军, 等. 广东省 4 种民间食用河豚鱼毒素检测[J]. 中国公共卫生, 2010 (7): 927-927.
    [11] 宫慧芝, 计融, 江涛, 等. 河豚毒素单抗 ELISA 检测试剂盒的研制[J]. 中国公共卫生, 2005, 21(12): 1423-1424.
    [12] 吴韶菊, 崔建洲, 宫庆礼. 河豚毒素的微生物起源[J]. 海洋科学, 2005, 29(10):81-85.
    [13] KODAMA M, SATO S, SAKAMOTO S, et al. Occurrence of tetrodotoxin in Alexandrium tamarense, a causative dinoflagellate of paralytic shellfish poisoning[J]. Toxicon, 1996, 34(10): 1101-1105.
    [14] YANG G, XU J, LIANG S, et al. A novel TTX-producing Aeromonas isolated from the ovary of Takifugu obscurus[J]. Toxicon, 2010, 56(3): 324-329.
    [15] 李秋芬, 徐怀恕. 河豚毒素及其微生物起源[J]. 海洋通报, 1994, 13(4): 86-91.
    [16] 池珍, 郑莺, 毛宁. 产河豚毒素 (TTX) 菌株 ZY-23 的分离与鉴定[J]. 微生物学通报, 2010, 37(2): 217-221.
    [17] 范延辉, 胡江春, 王书锦. 海洋细菌 B3B 产河豚毒素特性的鉴定及其发酵培养基优化[J]. 应用与环境生物学报, 2007, 13(3): 361-364.
    [18] 杨桂梅, 鲍宝龙. 河鲀和河鲀毒素之间关系的研究进展[J]. 上海水产大学学报, 2009, 17(6): 734-739.
    [19] 刘静, 杨桂梅, 鲍宝龙. 产河鲀毒素细菌水平转移相关基因的鉴定[J]. 上海海洋大学学报,2012,21(4): 509-515.
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引用本文

马廷龙,龚小玲,管哲成,鲍宝龙.云斑裸颊虾虎鱼体内各组织河鲀毒素的含量[J].上海海洋大学学报,2014,23(5):675-679.
MA Ting-long, GONG Xiao-ling, GUAN Zhe-cheng, BAO Bao-long. Analysis of the tetrodotoxin content of tissues in Yongeichthys criniger[J]. Journal of Shanghai Ocean University,2014,23(5):675-679.

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  • 收稿日期:2014-03-25
  • 最后修改日期:2014-05-18
  • 录用日期:2014-06-06
  • 在线发布日期: 2014-09-18
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